Composite

Part:BBa_K3590039

Designed by: Barbora Hrnčířová   Group: iGEM20_Brno_Czech_Republic   (2020-10-19)


Lysozyme derived from Gallus gallus fused with a dockerin derived from Clostridium thermocellum

This synthetic construct was designed to lyse the cyanobacterial cell wall while being connected to the ScafL.

At the 5' end of LysGG-DocCt there is the Pveg promoter followed by RBS R1. The CDS encodes several functional modules - SacB signal sequence for extracellular transport, His-tag for Western blot detection followed by lysozyme derived from Gallus gallus (LysGG). It has been shown that LysGG lyses the cyanobacterial cell wall. The LysGG module is followed by a linker that connects it to the dockerin domain of Clostridium thermocellum. This dockerin interacts exclusively with a cohesin domain originating from the same species, ensuring the attachment of this fusion protein to ScafL. At the 3' end of the CDS, there is a STOP codon.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 635
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
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